Biomedical Laboratory Science

Monday, August 8, 2016

Literature Review: Targeting Mutant Kinases

Small-Molecule Kinase Inhibitors Have Typically Been Designed To Inhibit Wild-Type Kinases Rather Than the Mutant Forms

Kinases have been the target of extensive research to identify drugs to treat a variety of diseases in which the wild-type kinase or a mutant kinase plays a crucial role. Kinase mutations frequently lead to an activated state where the kinase is always active and no longer tightly regulated. Considering resistance mutations are also important for kinase inhibitors. A frequent escape route is a mutation to the gatekeeper amino acid that blocks inhibitor binding. A variety of large screening panels have been developed that range from binding assays to enzyme assays, each with their unique pros and cons.

A variety of large screening panels have been developed that range from binding assays

to enzyme assays, each with their unique pros and cons. [nicolas_/Getty]

Source: genengnews

'Feeling Full' Hormone Increase in Seniors May Explain 'Anorexia of Aging'

Elderly adults often experience loss of appetite, resulting in weight loss and undernutrition. Now, researchers suggest this may be down to increased production of a hormone called peptide YY, which tells humans when they are feeling full.

Termed "anorexia of aging," loss of appetite is common among elderly adults, with around 15-20 percent of seniors experiencing unintentional weight loss as a result.

While loss of appetite in seniors can be driven by emotional issues, such as depression or grief, in many cases, no underlying cause can be found.

Previous research has suggested loss of appetite in the elderly may be down to reduced production of ghrelin - a hormone that tells humans when they are hungry.

However, the new study - conducted by Mary Hickson, professor of dietetics at Plymouth University in the United Kingdom, and colleagues - found the hormone peptide YY may be to blame.

Researchers identified increased production of the "feeling full" hormone PYY in elderly women,
which may explain why older adults often experience loss of appetite.

Source: medicalnewstoday

Next-Generation Sequencing and the Future of IVF

Medical laboratories play a vital role in helping patients achieve success with assisted reproductive technologies (ART) such as in vitro fertilization (IVF). Recent advances in genetic screening such as next generation sequencing (NGS) are revolutionizing how IVF will be performed in the future. NGS can sequence DNA and RNA more quickly than ever before. New applications like these technologies are raising hopes for improved IVF success rates to help patients achieve their dreams of building a family.

Preimplantation genetic screening

According to Centers for Disease Control and Prevention (CDC) estimates, one in eight couples have trouble achieving or sustaining a pregnancy, and approximately 7.4 million women in the United States have received help for infertility. The use of ART has doubled over the past decade.

Source: mlo-online

Funny Picture 18: "We're all out of anti-biotic cream, so I'd like to share with you about the healing properties of a dog's tongue."

Source: medicalnewstoday

See more funny pics!

Sunday, August 7, 2016

Science Finds a Super Easy Way to Lose Weight

Even if you order seemingly healthy dishes, your restaurant habit is likely derailing your weight loss efforts.

It’s been a long day, you’re tired, and the last thing you want to do is trudge to the grocery store and slave over the stove to make dinner. But hold up; don’t tell your hubby to meet you at your favorite restaurant just yet—especially if you’re trying to shed weight. According to a new study in the Journal of the Academy of Nutrition and Dietetics, nearly all restaurant entrées carry more calories than you should eat in a single meal. Even worse, a fair number of restaurants manage to load their meals with an entire day's worth of calories!

To come to this stomach-churning discovery, researchers from Tufts University visited both independent and chain restaurants across the country and purchased dozens of popular entrees from gyros and spaghetti to meatballs to burritos.

Eat This, Not That!

Source: msn.com/en-ca/health/wellness

How to Perform CPR

Forget mouth-to-mouth rescue breaths. This expert-backed technique is the correct way to perform this lifesaver

Sudden cardiac arrest occurs when the heart unexpectedly stops beating, which cuts off blood flow to the brain and other organs. If not treated, it can cause death within minutes.

In fact, only about 10 percent of people overall who experience it outside a hospital survive with their brain functions intact, a new study in JAMA found.

But early action can stave off the Grim Reaper: When sudden cardiac arrest victims first received CPR from bystanders, they were more likely to survive with favorable prognoses, the researchers discovered.

But the way you perform CPR has changed in recent years. You no longer have to put your mouth on the victim’s.

Sleep Apnea Triggers Pediatric Fatty Liver Disease Progression

Obstructive sleep apnea and low nighttime oxygen - which results in oxidative stress - may trigger progression of pediatric non-alcoholic fatty liver disease to non-alcoholic steatohepatitis.

Non-alcoholic fatty liver disease (NAFLD) describes the accumulation of fat in the liver of people who drink little or no alcohol. In some individuals with the condition, the accumulated fat causes inflammation and scarring in the liver, resulting in a more serious form of the disease called non-alcoholic steatohepatitis (NASH).

A disease of epidemic proportions, rates of NAFLD are increasing worldwide in both adults and children. NAFLD affects an estimated 30 percent of the population in Western countries and up to 9.6 percent of all children.

Around 38 percent of obese children are affected across the NAFLD spectrum, which includes isolated hepatic steatosis, non-alcoholic steatohepatitis, and cirrhosis.

A combination of OSA and low nighttime oxygen triggered NAFLD progression in obese adolescents.

Source: medicalnewstoday

Saturday, August 6, 2016

Appendicitis: Warning Signs and Early Symptoms

Appendicitis is a condition in which the appendix becomes inflamed. Its symptoms can become very uncomfortable, painful, and potentially life-threatening if left untreated.

Sudden appendicitis is the most common cause of acute abdominal pain requiring surgery in the United States. Additionally, more than 5 percent of the population develops appendicitis at some point.

Though it most commonly occurs between the ages of 10 and 30, appendicitis can develop at any age.

The appendix is a tube-shaped piece of tissue attached to part of the long intestine and severe pain is
often the first symptom of appendicitis. In most cases, it will begin near the belly button and surgery
may be needed to treat a case of appendicitis.

Source: medicalnewstoday

Resolving QC Failures

Most medical technologists and technicians, responsible for outputting test results as quickly and reliably as possible, hate it when their smooth flow of work is abruptly interrupted by an out-of-control QC rule flag. Suddenly they are faced with delayed reports, the prospect of a complicated technical investigation, and a litany of questions they must ask themselves, such as:

“Is the out-of-control condition real?”
“Do I really have a problem with the analytical system?”
“If I do have a problem, when did it start and how many patient samples are affected?”
“What should I do first?”
“What should I do next?”

The laboratory would have much less of a conundrum if it were using a new QC rule proposed in a 2012 Clinical Chemistry article.1 This rule allows, as part of its process control, the possibility of a second set of QC measurements when the first results are inconclusive.

Clinical Case Study: Red Brown Urine in a Patient with Chronic HIV Infection and Quadriparesis

A 42-year-old woman with chronic HIV infection presented with sudden onset of progressive limb weakness, leading to immobility within 4 days. This was preceded by severe abdominal pain, nausea, and vomiting for 2 days and episodes of confusion and agitation.

Six weeks prior, she had commenced highly active antiretroviral therapy (HAART), consisting of efavirenz/emtricitabine/tenofovir and cotrimoxazole for opportunistic infection prophylaxis. Additional history included constipation for 4 weeks and an admission for psychiatric symptoms 1 week before starting HAART. She had declined HAART when HIV infection was diagnosed 6 years earlier, but was successfully treated for multidrug-resistant tuberculosis.

Source: clinchem

Biomedical Laboratory Science